A SOLUBLE RIBONUCLEIC ACID INTERMEDIATE IN PROTEIN SYNTHESIS
نویسندگان
چکیده
منابع مشابه
A soluble ribonucleic acid intermediate in protein synthesis.
The cell-free rat liver system in which Cl*-amino acids are incorporated irreversibly into cY-peptide linkage in protein has been used in our laboratories for a number of years as a measure of protein synthesis. The essential components of this system are the microsomal ribonucleoprotein particles, certain enzymes derived from the soluble protein fraction, adenosine triphosphate, guanosine dior...
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The MO-formyltetrahydrofolate:methionyl soluble ribonucleic acid transformylase has been purified over 1500-fold from Escherichia coli B. Although not homogeneous at this stage, the enzyme was of sufficient purity to determine some physical characteristics. The sedimentation constant was 2.02 which would indicate a molecular weight of approximately 25,000. In addition to the substrates of the r...
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The total ribonucleic acid fraction of mature ovarian eggs of amphibia and fish is composed of two gross fractions, one soluble in cold 0.5 M perchloric acid and the other insoluble in the acid (1, 2). Furthermore, on prolonged treatment with mild alkali, about 80% of the acid is converted to mononucleotides, some 20% being recovered as oligonucleotide fragments, a few of which contain labile p...
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HeLa cells arrested in mitosis were obtained in large numbers, with only very slight interphase cell contamination, by employing the agitation method of Terasima and Tolmach, and Robbins and Marcus. Protein synthesis and RNA synthesis were almost completely suppressed in mitotic cells. Active polyribosomes were nearly absent in mitotic cells as compared with interphase cells treated in the same...
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LéJohn, Herbert B. (Purdue University, Lafayette, Ind.), and James S. Lovett. Ribonucleic acid and protein synthesis in Rhizophlyctis rosea zoospores. J. Bacteriol. 91:709-717. 1966.-The uniflagellate zoospores of Rhizophlyctis rosea display active motility and a high endogenous respiratory metabolism, but neither growth nor net ribonucleic acid (RNA) or protein synthesis can be measured by ord...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1958
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)77302-5